Systematic RNA interference reveals that oncogenic KRAS-driven cancers require TBK1 by Barbie David A, Tamayo Pablo, Boehm Jesse S, Kim So Young, Moody Susan E, Dunn Ian F, Schinzel Anna C, Sandy Peter, Meylan Etienne, Scholl Claudia, Fröhling Stefan, Chan Edmond M, Sos Martin L, Michel Kathrin, Mermel Craig, Silver Serena J, Weir Barbara A, Reiling Jan H, Sheng Qing, Gupta Piyush B, Wadlow Raymond C, Le Hanh, Hoersch Sebastian, Wittner Ben S, Ramaswamy Sridhar, Livingston David M, Sabatini David M, Meyerson Matthew, Thomas Roman K, Lander Eric S, Mesirov Jill P, Root David E, Gilliland D Gary, Jacks Tyler, Hahn William C in Nature (2009). PubMed

Abstract

The proto-oncogene KRAS is mutated in a wide array of human cancers, most of which are aggressive and respond poorly to standard therapies. Although the identification of specific oncogenes has led to the development of clinically effective, molecularly targeted therapies in some cases, KRAS has remained refractory to this approach. A complementary strategy for targeting KRAS is to identify gene products that, when inhibited, result in cell death only in the presence of an oncogenic allele. Here we have used systematic RNA interference to detect synthetic lethal partners of oncogenic KRAS and found that the non-canonical IkappaB kinase TBK1 was selectively essential in cells that contain mutant KRAS. Suppression of TBK1 induced apoptosis specifically in human cancer cell lines that depend on oncogenic KRAS expression. In these cells, TBK1 activated NF-kappaB anti-apoptotic signals involving c-Rel and BCL-XL (also known as BCL2L1) that were essential for survival, providing mechanistic insights into this synthetic lethal interaction. These observations indicate that TBK1 and NF-kappaB signalling are essential in KRAS mutant tumours, and establish a general approach for the rational identification of co-dependent pathways in cancer.

[ hide abstract ]

Discussed In Paper